摘要
采用兔下腹部游离皮瓣模型，通过结扎股动脉造成皮瓣缺血１２小时，在吻合血管恢复血运之前，分别用生理盐水和超氧化物歧化酶（ＳＯＤ）灌注皮瓣，分别测定正常、缺血、用药物灌注后和血液再灌注后皮肤中ＳＯＤ的活性和丙二醛的含量，并记录各组皮瓣成活面积，结果表明，游离皮瓣缺血再灌注时有自由基产生，自由基对皮瓣有损伤作用，ＳＯＤ局部灌注可提高缺血游离皮瓣的成活率。
erfusion of free flaps from groin of rabbits,after 12 hours of complete ischemia, with su-peroxide dismutase(SOD)、an oxygen free radical scavenger.would significantly increase thesurvival rate of these flaps from 18. 75%to 75%in the control group.Tissue levels of SOD andmalonydialdehyde(MDA, an end produet of lipoperoxidation)were measured before ischemia,after ischemia but before reperfusion,and 60 minites after reperfusion.In untreated flap,after12 hours' ischemia,the SOD content of skin decreased significantly as compared with the SODcontent before ischemia,and reperfusion furtber decreased SOD activity.while the concentrationof MDA increased after ischemia and further inereased after reperfusion In the treated ftaps , theconcentration of SOD was not decrease and MAD not inci eased after reperfusion. There was anegative correlation between the values of SOD and M DA。 These findings suggested that freeoxygen radicals played an important role in the free flap ischemia reperfusion injury. SOD couldincrease the survival of ischemic free-flaps by rcducing lipoperoxidation.The results had signifi-cant clinical impllcations with regard to organ preservation and transplantation.
出处
《中国修复重建外科杂志》
CAS
CSCD
1995年第2期104-107,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
皮瓣
缺血
再灌注损伤
自由基
超氧化物歧化酶
Skin flap
Ischemic reperfusion injury
Frce radicals
Superoxide dismutase.
