摘要
本试验初步研究了双歧杆菌ＤＭ９２２７菌株及乳杆菌ＤＭ８１２１菌株对大鼠肠菌群失调的调整作用。将含有红霉素及链霉素的抗生素溶液给２０只大鼠灌胃，灌胃量为每种抗生素２０００ｍｇ／ｋｇ／日，连续３天。然后将动物分为双歧杆菌及乳杆菌菌液（ＢＬ）灌胃组及ＰＢＳ灌胃对照组。前者给每只大鼠灌胃含有双歧杆菌ＤＭ９２２７株及乳杆菌ＤＭ８１２１株各为１．５×１０９／ｍｌ的菌液２ｍｌ，每日２次，连续５天；后者以ＰＢＳ灌胃。分别检测抗生素灌胃前、灌胃后，ＢＬ菌液灌胃后及ＰＢＳ灌胃后大鼠的粪菌群成员：肠杆菌、肠球菌、乳杆菌及双歧杆菌。结果表明，抗生素灌胃后诱导产生了大鼠的肠菌群失调，表现出粪便中肠杆菌下降（Ｐ＜０．００１）、肠球菌升高（Ｐ＜０．０５）、乳杆菌及双歧杆菌下降（Ｐ＜０．００１）。ＢＬ菌液能明显纠正肠菌群的失调状态，使上述４种菌恢复到正常水平。
This study investigated the adjustment of intestinal
dysbacteriosis byBifidobacterium and Lactobacillus in rats.20 Wistar rats were employed,each
was orally treated with 200mg/kkg/day erythromycin and the sameamount of streptomycin for 3
days,After that,10 rats of the experimen-tal group were orally infused with 2ml living bacterial
Suspensionttwiee a day for 5 days, which contained 1.5 × 109/ml BifidobacteriumDM9227
strain and 1.5×109/ml Lactobacillus DM8121 strain,The other 10rats were given PBS as a
control group.The rats′fecal flora includingEnterobacter, Enterococcus,Lactobacillus and
Bifidobacterium weredetected. The results showed that the intake of antibiotics could
induceintestinal dysbacteriosis in rats,presenting a remarkably increasedEnterococcus(P<0.05)
and lowered Entcrobacillus(P<0.001), Lac-tobacillus(P<0.001)and Bifidobacterium (P<0.001).The
living bacterial suspension could effectively adjust the disordered flora to normallevel.
出处
《中国微生态学杂志》
CAS
CSCD
1995年第2期32-34,共3页
Chinese Journal of Microecology
关键词
肠疾病
双歧杆菌
乳杆菌
肠菌群失调
Bifidobacterium Lactobacillus Intestinal dysbacteriosis
