摘要
本文采用放射自显影受体显示技术，对豚鼠肺组织的Ｍ胆碱能受体进行了定位和定量研究，并分析了卵白蛋白过敏性哮喘状态下其分布密度的变化。结果表明：豚鼠肺组织内的Ｍ受体主要集中在各级支气管平滑肌层上，其中文气管平滑肌上的Ｍ受体银颗粒密度最大，依次的分布密度为小支气管平滑肌＞细支气管平滑肌＞支气管粘膜上皮和腺体＞末梢肺泡区，血管壁平滑肌上的Ｍ受体银颗粒数几乎为零。同对照组相比哮喘动物肺组织的Ｍ受体银颗粒数有区域性增高变化；其中小支气管平滑肌和细支气管平滑肌上的Ｍ受体银颗粒数分别比对照组高出２１．０３％和６８．７８％（Ｐ＜０．０１），支气管粘膜上皮的Ｍ受体也比对照组高出３７．１３％（Ｐ＜０．０５）。同时证明哮喘发作时肺组织的ｃＧＭＰ含量与Ｍ受体的变化相一致。由此提示，哮喘发作中的小气道阻力增强与Ｍ受体数量的增加有直接的内在联系。
The distribution of M-cholinergic receptors along the airway in guinea pig and the local changes of M-receptors during asthma onset was analyzed by autoradio-graphy and the pathogenesis of asthmas was explained. Light microscopic observation showed that M-receptors were localized mainly cn the smooth muscle layer of bronchi and bronchioles of the animals. The highest density of labeling grains of M-receptor was found in bronchial smooth muscle, the second and third were small bronchial and bronchiolar smooth muscle respectively.There are only a few M-receptor grains in alve-olar area and almost no in vascular smooth muscle.In asthmatic guinea pigs, the M-receptor density was inereased regionally.Comparing with those of control group, the receptor labeling grains were increased 21.03% and 6.78% in bronchial and bronchio-lar smooth muscle respectively(P<0.01 ). The M-receptor grains on bronchial mucosalepithelium was increased 37.13%(P<0.05 ).The cGMP content of asthmatic guinea piglung was significantly higher than that of control group.The data suggested that in-creased M-receptors might be one of the causes which lead to the spasm of small air-ways during asthmatic onset.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1995年第1期11-15,共5页
Chinese Journal of Pathophysiology
