摘要
用聚丙烯酰胺凝胶电泳技术，对内蒙古自治区三个不同地理位置的少数民族自治旗及一个城市汉族对照区秋冬季采集的新生儿胎便及婴幼儿急性腹泻便进行轮状病毒ＲＮＡ基因组特点分析。共检测胎便标本１４６份，腹泻便标中１１６份。结果表明：几个少数民族地区流行的轮状病毒均以Ａ组第Ⅱ业组为士，并在一个地区有多种差异电泳型存在。新生儿胎便中可检出轮状病毒并显示了特征性Ａ组轮状病毒ＲＮＡ基因型。胎便小轮状病毒检出率高的地区，腹泻便检出率也相应高，轮状病毒的检出率与民族、地理位置尤明显关系，而与当地的卫生条件及地形有关。阳性标本使用Ａ组单克隆抗体酶标免疫试剂进一步证实。
is paper reports that the HRV detection results from different ethnic minorities in In-ner Mongolia,China,146 meconium and 1 16 acute diarrhea samples were cullected from hos-Ditalized newborns and infants during winter diarrhea period in 1 993 and were examined byPAGE and McAb VP_6 ELISA.The results showed that different ethnic minority areas hadthe same predominant electropherotype,but different subtypes.HRV detected in newbornmeconium had clear genotypes of HRV group A and all belong to subgroup Ⅱ(long electro-pherotype).High HRV positive rate was found in certain area,but there was no correlationsbetween either ethnic races or geographical locations.According to epidemic investigation,lo-cal environmental hygiene level was the main infection factor of the disease.This conclusionwas further confirmed by McAb VP6 ELISA.
出处
《中国病毒学》
CSCD
1995年第3期191-196,共6页
Virologica Sinica
基金
卫生部青年科学基金
