摘要
将茎瘤芥“棒棒菜”（Ｂｒａｓｓｉｃａｊｕｎｃｅａｖａｒ．ｔｕｍｉｄａｃｖ″Ｂａｎｇｂａｎｇｃａｉ″）下胚轴离休培养的再生芽，用１２０～４０Ｇｙγ射线照射后，在原培养基（ＭＳ＋ＮＡＡ０．２ｍｇ／ｌ＋ＢＡ２．０ｍｇ／ｌ）上培养１周，转到加Ｓ－（２－氨乙基）－Ｌ－半胱氨酸（ＡＥＣ）的原培养基中，经过连续７代选择，获得了抗ＡＥＣ的变异体植株。其蛋氨酸和精氨酸含量分别为对照的１．８４倍和３倍，赖氨酸比对照略有增加，总氨基酸含量为对照的１．４５倍。
The regenerated shoots derived from stem tumour mustard cultivar ″Bangbangcai″,Brassica juncea var.tumida cv ″Bangbangcai″,were radiated by γ ray(￣(60)Co)at 120 GY,80 Gy or 40 GY. After the radiated shoots were cultured in original medium, MS+ 0.2 mg/1 NAA+2.0mg/l BA, for 7 days,they were transfered to the medium supplemented with S-2-aminoethyl-cystein(AEC).Variant plants resistant to AEC were achieved through selection and culture for 7 generations in succession. Content (mg/100 g FW)of methionine,arginine is as much 1.8 and 3.0 times as that of the CK respectively. Total amino acids and lysin are also increased as compared with the CK.
出处
《西南农业大学学报（自然科学版）》
CSCD
1995年第2期98-101,共4页
Journal of Southwest Agricultural University
基金
四川省科委和重庆市科委博士基金
关键词
芥菜
棒棒菜
离体培养
半胱氨酸
变异体
indian mustard
Brassica juncea var.tumida cv "Bangbang cai″
cul-ture in vitro
