摘要
使用２．１μｍ的单分散无孔硅胶作为基体，表面化学键合Ｃ＿８和Ｃ＿（１８）非极性固定相，并在这种键合相和相关的大孔硅胶上考察了蛋白质分离的色谱性能。实验结果表明，梯度冲洗下该固定相能使核糖核酸酶、胰岛素、溶菌酶、牛血清蛋白和卵清蛋白得到好的分离，分析速度快、效率高，为生物大分子的高效液相色谱分离开辟了另一条途径。
n this work,the bonding of C_8 and C_(18) to 2.1μm monodisperse non-porous silica for the preparation ofnovel hydrophobic packings has been studied.The chromatographic properties of the non-porous packingsand large pore packings were examined and compared.The results showed that good revesed-phase bondedsilica packings can be prepared.Under gradient elution. proteins(RNase,Ins,Lys,BSA and Ova)wereseparated within 15min successfully. It is suggested that the non-porous packings are a better alternative forthe biopolymer separation.
出处
《色谱》
CAS
CSCD
北大核心
1995年第5期361-364,共4页
Chinese Journal of Chromatography
关键词
高效液相色谱
无孔硅胶
蛋白质
色谱分离
high performance liquid chromatography,biopolymer separation, non-porous silica reversed-phase bonded stationary phase,protein
