摘要
目的:观察电针治疗对成年大鼠脑缺血后缺血侧神经干细胞特异性标记物巢蛋白表达的影响。方法:实验于2003-06/2004-03在广州中医药大学实验动物中心进行。取清洁级SD雄性大鼠72只,随机分为两组,即模型组(n=36)与电针组(n=36),均采用开颅热凝闭法制作大鼠局灶性脑缺血模型,按缺血时间3,7,14和21d将模型组与电针组又各分为4组,即8个亚组(n=9)。对电针组采用1寸毫针针刺大椎、百会,联接G6805-1型电针治疗仪,选择5～10Hz频率的等幅疏密波,刺激强度以大鼠身体轻微颤动为宜,持续30min。模型组大鼠只予以同等条件抓取,但不实施电针治疗处理。观察不同时相脑缺血情况,采用巢蛋白免疫组化法记录大鼠巢蛋白表达阳性细胞的数量,其数量增加表明神经干细胞的增殖。结果:①模型组脑缺血后不同时相(3,7,14和21d)巢蛋白在缺血侧脑皮质、海马齿状回均有阳性表达细胞犤(5.20±1.70,14.16±3.95,9.26±3.29,6.70±2.27),(13.58±2.33,20.34±2.73,13.12±2.25,8.64±1.56)犦,其中7d的阳性细胞数量比其他时相明显增加,差异显著(P<0.05);②电针组在不同时相的巢蛋白阳性细胞数量均较同时相模型组有明显的增加(缺血侧脑皮质:11.50±3.39,26.40±4.65,18.16±3.03,12.66±2.57;海马齿状回:20.04±3.60,29.26±3.16,18.78±2.24,13.52±2.10),差异显著(P<0.05)。结论:电针具有促进大鼠脑缺血后巢蛋白在缺血侧阳性表达保持在较高水平的作用。
AIM： To investigate the effect of electroacupuncture（EA） on the expression of nestin （specific marker of neural stem cells） in ischemic sides of adult rats with cerebral ischemia. METHODS： The experiment was conducted in the Center for Experimental Animals, Guanghou University of Traditional Chinese Medicine between June 2003 and March 2004. Seventy-two male SpragueDawley rats of SPF grade were selected and divided into model group （n=36） and EA group（n=36）. All the rats were induced by opening cranium hot coagulate to establish the model of focal cerebral ischemia. Then each group was subdivided into 4 groups according to the different ischemia courses（3,7,14 and 21days）, i.e., 8 groups with 9 rats in each group. Rats in the EA groups were stimulated by a needle of 1 inch at “Dazhui”and “Baihui”points followed by EA using G6805-1 EA apparatus（constant sparse-dense amplitude of 5 to 10 Hz, stimulus intensity suitable for slight shake of rat） for 30 minutes. Rats in the model groups were only grasped under the same condition as in the EA groups, but not by EA treatment. lmmunohistochemical staining of nestin was used to record the number of positive nestin cells in rats at different time after cerebral ischemia, and increase of the number meant proliferation of neural stem cells. RESULTS：①In the model groups,there were positive nestin cells in the cortex（5.20±1.70, 14.16±3.95, 9.26±3.29, 6.70±2.27）and hippocampus （13.58±2.33, 20.34±2.73, 13.12±2.25, 8.64±1.56） at 3,7,14 and 21 days after cerebral ischemia, especially the number of positive cells on the 7^th day was increased significantly（P 〈 0.05）.②）In the EA groups, the number of positive nestin cells in the cortex（11.50±3.39, 26.40±4.65, 18.16±3.03, 12.66±2.57） and hippocampus （20.04±3.60, 29.26±3.16, 18.78±2.24, 13.52±2.10） was significantly more than that in the model groups at 3,7,14 and 21 days（P 〈 0.05）. CONCLUSION： EA can promote and keep high expression of the positive nestin cells in ischemic sides of rats with cerebral ischemia.
出处
《中国临床康复》
CSCD
北大核心
2005年第26期89-91,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省自然科学基金项目(31458)~~
