摘要
目的建立测定甲砜霉素胶囊含量的方法.方法采用高效液相色谱法,选用Agilent Eclipse XDB-C8柱 (4.6 mm×250 mm,5 μm), Lab Alliance C8保护柱(4.6 mm×10 mm),流动相为乙腈-水(25∶75),流速1.0 ml· min-1,检测波长226 nm,进量样20 μl, 柱温为室温.结果线性范围为10～400 μg· ml-1(r = 0.9999),最低检测限2.0 ng,回收率在99.6%～101.1%之间,RSD为0.85%～3.48%(n=5).结论所用方法简便、灵敏,结果准确可靠,可用于甲砜霉素胶囊的质量控制.
OBJECTIVE To determine the content of Thiamphenicol capsule. METHODS An HPLC method was performet on an Agilent E-clipse XDB- C8 columm（4.6 mm×250 mm,5μm）with the mobile phase consisted of acetonitrile- water （25：75）. The flow rate was 1.0 ml·min^-1 and detection wavelength was at 226nm . The injection vohmle was 20μl and external standard method was used.RESULTS The linear range of this method was 10 - 400μg· ml^-1 （ r = 0. 9999） with the limit of detection of 2.0 ng . The average recovery of Thiamphenicol was 99.6% to 101.1%,with RSD of 0.85% to 3.48%（n = 5）. CONCLUSION The method is simple,sensitive,accurate and can be used for the determination of Thiamphenicol capsule.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2005年第4期353-354,共2页
West China Journal of Pharmaceutical Sciences
基金
浙江省分析测试基金资助项目(编号:04083)
