摘要
目的探讨莪术油对小鼠结肠癌抑制作用及机制。方法采用MTT法检测莪术油对癌细胞的生长抑制作用;建立荷瘤鼠模型,观察荷瘤鼠的肿瘤大小及生存时间;TUNEL法检测原位细胞凋亡。结果莪术油体外可明显抑制小鼠CT26结肠癌细胞生长,其浓度与细胞生长抑制率成正比;莪术油明显抑制肿瘤生长(P<0·05);莪术油组对荷瘤鼠明显延长平均生存时间(P<0·05);莪术油明显促进荷瘤鼠结肠癌细胞的原位凋亡(P<0·05)。结论莪术油能够诱导机体抗肿瘤反应,促进细胞凋亡可能是其主要作用机制。
Objective： To investigate the the anti-tumor effect with curcuma aramatica oil in adenocarcinoma and mechanism in mice. Methods： The inhibiting effect was detected by MTT. The model of colorectal adenocarcinoma wasestablished in mice. Comparative analysis between control and experimental groups on the survival of mice and the growth of tumor. Apoptosis of adenocarcinoma cells was evaluated by the method of TUNEL. Results： Curcuma aramatica oil inhibited the growth of CT26 cells significantly in vivo concerntration dependent. The growth of tumor was suppressed by curcuma aramatica oil （ P 〈 0.05 ）. The average survival time was far longer than control group （ P 〈 0.05 ）. The index of apoptosis （AI） was singnificantly higher than that of conrol group （ P 〈0.05） .Conclusion： Curcuma aramatica oil can induce the anti-tumor effect, perhaps promoting apoptosis is the main mechanism.
出处
《泰山医学院学报》
CAS
2005年第2期89-91,共3页
Journal of Taishan Medical College
关键词
莪术油
结肠癌
细胞凋亡
作用机制
curcuma aramatica oil
colorectal adenocarcinoma
apoptosis
