摘要
选择绵羊1号染色体上的9个微卫星标记,采用父系半同胞家系群体(共387个个体)构建凉山半细毛羊1号染色体遗传连锁图。建立的资源参考家系通过20个微卫星标记进行了系谱确证。结果表明,9个标记的等位基因数变化范围为5～15,杂合度在0.202～0.831之间,平均杂合度为0.617,各标记的平均多态信息含量PIC=0.604。构建的凉山半细毛羊1号染色体遗传连锁图总长度311.0cM,与美国肉畜中心(USDA)和国际绵羊作图中心(IMF)构建的绵羊1号染色遗传连锁图结果基本一致。可用于下一步的QTL定位研究。
The 9 microsatellite markers on chromosomes 1 were used to construct a linkage map of Liangshan semiwool sheep with a 387 members half-sib pedigrees after paternity testing. The results were as follows： the number of alleles for 9 markers was from 5 to 15; the heterozygosity was from 0. 202 to 0. 831, the average heterozygosity was 0. 617 and the polymorphic information content （P/C） was 0.604. Length of the linkage map of chromosome 1 we built was 311.0 cM which is in consistent with the sheep linkage map of USDA and IMF, and can be used in Quantitative Trait Loci searching in Liangshan semi-wool sheep.
出处
《遗传》
CAS
CSCD
北大核心
2005年第4期575-578,共4页
Hereditas（Beijing)
基金
德国联邦科技与教育部(BMBF)资助(项目代号:CHN00316)~~
关键词
凉山半细毛羊
微卫星标记
连锁图
Liangshan semi-wool sheep~ linkage map~ microsatellite markers
