摘要
目的:观察激光透穴离子导入法对大鼠大脑中动脉缺血再灌注模型损伤的影响。方法:将SD大鼠 随机分为正常组、假手术组、模型组、电针组、激光透穴离子导入组。采用线栓法制备大鼠大脑中动脉缺血再 灌注模型,电针组和激光透穴离子导入组在造模后即刻、12h、24h分别进行3次治疗,并观察各组的神经功 能缺损积分和体质量的变化,检测脑组织中的超氧化物歧化酶(superoxidedismutase,SOD)的活性及丙二 醛(malondialdehyde,MDA)的含量。结果:与模型组比较,激光透穴离子导入组的神经功能缺损积分明显 降低,体质量下降和SOD活性下降的幅度明显减小,MDA含量明显减少。结论:激光透穴离子导入法具有 保护脑缺血再灌注损伤的作用,其作用机制可能与提高SOD活性,减轻氧化物对机体的损伤有关。
Objective： To investigate the effects of low-level laser irradiation on acupuncture points combined with iontophoresis against brain damage after middle cerebral artery occlusion （MCAO） in rats.Methods： Sixty-nine SD rats were randomly divided into five groups, including normal group, sham operation group, model group, electro-acupuncture group and low-level laser irradiation on acupuncture points combined with iontophoresis group （LLL1 group）. The cerebral ischemia-reperfusion （I/R） model was established by thread embolism of middle cerebral artery. The rats in the LLLI group, as well as the electro-acupuncture group were given treatment as soon as the occlusion finished （0 hour） and 12, 24 hours after the occlusion.We observed the changes of neurological deficit scores and the body weight of the rats at different time. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the rat＇s brain tissue were tested. Results： The neurological deficit score of the LLLI group was significantly lower than that of the model group. The body weight and the activity of SOD of the rats decreased slightly, and the content of MDA decreased significantly after the treatment. Conclusion： The low-level laser irradiation on acupuncture points combined with iontophoresis can prevent focal cerebral ischemia-reperfusion injury. One of its mechanisms may be increasing the activity of SOD and decreasing the damage of the oxidation products to the body.
出处
《中西医结合学报》
CAS
2005年第2期128-131,共4页
Journal of Chinese Integrative Medicine
基金
上海市教委科研基金资助项目(No.03ck15)
关键词
激光疗法
离子透入法
脑缺血
超氧化物歧化酶
丙二醛
laser therapy
iontophoresis
brain ischemia
superoxide dismutase
malondialdehyde
