摘要
利用０．１％盐酸升汞液消毒１．５ｍｉｎ或用３．７５％次氯酸钠消毒７ｓ，可以在越冬病标样上分离到纹枯病菌丝。利用无菌水漂洗菌核再加培养也可以分离到纹枯病菌丝。土壤中纹枯病菌丝可用打孔试管埋管法分离。病菌扩繁以小米培养基为好。对稻株接种可以将接种物直接置于叶鞘深处，不必保湿。
Using 0.1% solution of hydrochloric acid and mercuric chloride to disinfect the affected samples for 1.5 minutes or using 3.75% solution of sodium subchlorate for 7 seconds can isolate the the mycelia of Rice sheath blight from affected sample which past winter. Using sterilized water to wash the sclerotia of T.cucumeris.The mycelia of T.cucumeris in the soil can be isolated by burying the testtubes which have several small holes.
出处
《吉林农业大学学报》
CAS
CSCD
1995年第1期10-15,共6页
Journal of Jilin Agricultural University
关键词
水稻
纹枯病菌
分离
培养
人工接种
Thanatephorus cucumeris(Frank) Donk
isolate
cultivate
artificial inoculation
