摘要
本文介绍一种胃动素RIA。采用合成的猪胃动素与牛甲状腺球蛋白偶联,免疫新西兰白兔,成功地获得了一高质量抗血清,亲和常致达3.6×1010L/M(Scatchard作图法)。测定36例胆囊切除术后病人血浆胃动素浓度的动态变化,并前瞻性随机对照观察了大承气汤冲剂对其影响及促进病人术后胃肠道运动功能恢复的治疗作用。结果提示:大承气汤冲剂具有促进术后病人胃肠道运动功能恢复的作用,而这一作用是通过体液调节因素—胃动素的参与实现的。
he development of a radioimmunoassay for motilin is intreduced. The antisera to syntheticporcine motilin conjugated with bovine thyroglobulin was raised in rabbits,The affinity constantof the antisera was 3.6×1010L/M(derived from Scatchard chart). The dynamic change of plas-ma motilin was observed and the effects of Da Cheng Qi decoction on the hormone and gastroin-testinal movement was evaluated in 36 patients after cholecystectomy and controls。 It was sug-gested that Da Cheng Qi decoction could improve the gastrointestinal movement recovery whichmight be mediated by motilin.
出处
《中国中西医结合外科杂志》
CAS
1995年第3期153-156,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine