摘要
目的:观察吗啡成瘾时小鼠血清及肝脏超氧化物歧化酶(SOD)和肝脏丙二醛(MDA)含量的变化。方法:将小鼠分为4组,分别为对照组、吗啡成瘾组、纳络酮诱发戒断组和自然戒断组,每组12只。对照组注射生理盐水,另外3组按100mg/(kg·d)注射盐酸吗啡,均注射11d,在末次注射后对对照组、吗啡成瘾组、纳络酮诱发戒断组小鼠处死收集血清,自然戒断组在末次吗啡注射24h后处死并收集血清。各组小鼠处死后立即取肝脏,称重,加入生理盐水匀浆后取上清液进行测定。采用羟胺比色法对SOD活性进行测定,采用硫代巴比妥酸比色法进行MDA测定。结果:吗啡成瘾组,纳络酮诱发戒断组及自然戒断24h组小鼠血清及肝脏SOD水平低于对照组水平(P<0.05),肝脏MDA高于对照组水平(P<0.05)。结论:吗啡成瘾和戒断时血清及肝脏SOD水平降低,MDA水平升高。
Aim: To detect the levels of serum and liver SOD,MDA levels in morphine addicted mice.Methods: Mice were divided into four groups(12 in each group). One group was injected with normal saline at the dose of 0.5 ml per day for 11 days as control. The other three groups were injected morphine hydrochloride at the dose of 100 mg/(kg·d) for 11 days to induce morphine-addicted model. Serum was isolated in control group, morphine addicted group, and morphine abstinence group at 6 h after the last injection. Serum was collected in the spontaneous abstinence group at 24 h after the last injection. The liver of each mouse was weighted and homogenized with cold saline and centrifugated to get supernatant for future determination. SOD was determined using hydroxylamine colorimetry.MDA was determined using sulfide barbital acid method. Results: The serum and liver SOD in morphine addicted group, morphine abstinence group,and spontaneous abstinence group were lower than that of control group, respectively (P<0.05 or 0.01). The liver MDA level in morphine addicted group, morphine abstinence group,and spontaneous abstinence group were higher than that of control group (P<0.05).Conclusion: The serum and liver SOD levels are decreased, and MDA is increased after morphine addiction and morphine abstinence.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第4期694-695,共2页
Journal of Zhengzhou University(Medical Sciences)
