摘要
目的探讨三氧化二砷(As2O3)对体外培养的人类成神经胶质瘤细胞株U251MG的作用及其机制。方法应用倒置相差显微镜、四甲基偶氮唑蓝法、流式细胞仪,分别对不同浓度加药组及对照组U251细胞的存活、形态学改变、细胞DNA含量的分布进行观察和测定。结果0.5～4μmol/L的As2O3可明显抑制U251的增殖。流式细胞仪分析显示,加药组在G1期细胞前均出现亚二倍体峰,且G0/G1期细胞减少,诱导细胞凋亡。结论As2O3能抑制体外人胶质瘤细胞株U251的增殖,有望用于神经胶质瘤的治疗。
Objective It is to discuss the action and mechanism of As_2O_3 on human glioblastoma cell line U251 cultivated in vitro. Methods The survivorship, morphology change, distribution of cell DNA content of U251 cells in the groups given medicine of different concentration and control group were observed and measured with inverted phase contrast microscope, MTT method and flow cytometry. Results The concentration of 0.5～4 μmol/L of As_2O_3 could obviously inhibit the proliferation of U251. Flow cytometry showed that the peak of hypodiploid occurred before G1 stage in every group given medicine, and cells decreased with apoptosis of induction cells in G_0/G_1 stage. Conclusion As_2O_3 can inhibit the proliferation of human glioblastoma cell line U251 in vitro. It can be used to treat glioma.
出处
《现代中西医结合杂志》
CAS
2005年第14期1829-1830,共2页
Modern Journal of Integrated Traditional Chinese and Western Medicine
