摘要
目的 探讨人巨细胞病毒(HCMV)感染与急性冠状动脉综合征(ACS)患者血清sP 选择素、肿瘤坏死因子(TNF α)的变化及相关性。方法 采用酶联免疫吸附技术检测79例ACS患者、30例稳定性心绞痛(SA)患者和30例正常对照组血清HCMV IgM、HCMV IgG、sP 选择素和TNF α的水平。结果 (1)在ACS、SA及正常对照组中,血清HCMV IgM、IgG的阳性率分别为30 4 % (2 4 79)、10 0 %(3 30 )和6 7% (2 30 ) ;86 1% (6 8 79)、80 0 % (2 4 30 )和5 3 3% (16 30 )。HCMV IgM的阳性率在ACS组中明显高于SA组和对照组(P <0 0 1) ,HCMV IgG的阳性率在ACS和SA组中明显高于对照组(P <0 0 1)。(2 )与SA组和对照组相比,ACS组中血清sP 选择素和TNF α水平显著升高,分别为(15 2 0 0±112 7)和(14 81 0±10 9 1)pg ml比(6 4 37 3±6 6 6 9)pg ml,(2 7 3±13 7)pg ml和(2 8 1±11 3)pg ml比(5 6 2±18 4 )pg ml,组间差异有统计学意义(P <0 0 1)。随着冠状动脉(冠脉)病变程度的增加,ACS中急性心肌梗塞(AMI)组与不稳定性心绞痛(UA)组相比,sP 选择素和TNF α水平显著升高(P <0 0 1) ,而SA组与对照组间差异无统计学意义(P >0 0 5 )。(3)ACS组中HCMV IgM阳性患者血清中sP 选择素和TNF α水平较HCMV IgM阴性患者明显升高(P <0
Objective To explore the changes and relationship between serum soluble P-selectin, tumor necrosis factor-alpha (TNF-α) in coronary heart disease patients with acute coronary syndrome (ACS) and human cytomegalovirus (HCMV) infection. Methods The levels of circulating soluble P-selectin, TNF-α, HCMV-IgM and HCMV-IgG were determined by enzyme-linked immunosorbent assay (ELISA) in 79 cases for ACS group, 30 cases for stable angina (SA) group and 30 healthy control cases. Results (1) The serum positive rate of HCMV-IgM and HCMV-IgG in the ACS, SA and healthy control groups were 30.4%(24/79), 10.0%(3/30) and 6.7%(2/30); 86.1%(68/79), 80.0%(24/30) and 53.3%(16/30), respectively. Positive rate of HCMV-IgM in the ACS was higher than those in SA and healthy control groups (P<0.01), positive rate of HCMV-IgG in the ACS and SA groups were higher than that of the healthy control group (P<0.01). (2) Compared with the SA group and healthy control group, the levels of the serum soluble P-selectin and TNF-α were significantly higher in patients with ACS [(6 437.3±666.9) pg/ml vs. (1 520.0±112.7) pg/ml and (1 481.0±109.1) pg/ml, (56.2±18.4) pg/ml vs. (27.3±13.7) pg/ml and (28.1±11.3) pg/ml], respectively, P<0.01). The AMI group, compared with the UA group in the ACS group, had significantly higher levels of the serum soluble P-selectin and TNF-α (P<0.01).Compared with the SA group, the levels of the serum soluble P-selectin and TNF-α were not significantly different in healthy control group. (3) The levels of the serum soluble P-selectin and TNF-α in HCMV-IgM positive patients were significantly higher than the HCMV-IgM negative patients in the ACS group (P< 0.01). Conclusion The chronic infection with HCMV might injure endothelial cells that subsequently contribute to the formation and progression of atherosclerosis, the acute infection with HCMV may induce increased serum levels of soluble P-selectin and TNF-α that might participate in acute coronary events.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2005年第2期149-151,共3页
Chinese Journal of Experimental and Clinical Virology
基金
陕西省科学技术研究发展计划项目 (2 0 0 1K10 G7)
