摘要
作者构建了一个能表达ｃ－ｅｔｓ－２、ｃ－ｍｙｃ及Ｎ－ｒａｓ三个癌基因联合反义ＲＮＡ的重组逆转录病毒载体，经病毒包装细胞ＰＡ３１７包装成假型逆转录病毒，利用此病毒成功地感染了人肝癌细胞株ＳＭＭＣ－７７２１，经Ｇ４１８筛选得到Ｇ４１８抗性细胞，基因组ＤＮＡ杂交结果表明重组病毒稳定地整合入７７２１细胞基因组中。ＲＮＡ杂交结果表明转化细胞中有较高水平的联合反义ＲＮＡ表达。初步结果表明，反义ＲＮＡ使７７２１细胞生长速率下降约７０％，软琼脂集落形成能力及裸鼠致瘤能力显著下降。这一结果表明，针对多个癌基因的联合反义ＲＮＡ可能给肿瘤基因治疗提供新的途径，有进一步探索的价值。
Abstract A recombinant retroviral vector was constructed which expressed antisense RNA of c-ets-2,c -myc and N-ras.The pseudotype virus was packaec and rescued by transfection in PA317 cells and used to infect human hepatoma cell line SMMC-7721.After selection with G418,resistant colonies were obtained. Stable integration of retrovirus in infectants was shown bv Southernhybridization of genomic DNA and the presence of antisense RNA was detected by RNA dot blot hybribization. It was demonstrated that the antisense RNAs did inhibit the growth of humanSMMC-7721 hepatoma cells.The ability to form colony in soft agar and tumorigenicity in nudemicr of SMMC-7721 were significantly suppressed by the antisense RNAs. The result implicatesthe potential value in future cancer gene therapy.
出处
《中华肿瘤杂志》
CSCD
北大核心
1994年第4期243-246,共4页
Chinese Journal of Oncology
