摘要
运用Ｗｅｓｔｅｒｎ印迹方法，将甲状腺、眼外肌、眼球周围结缔组织的细胞浆与细胞膜粗提抗原以及骨骼肌、肝脏细胞膜粗提抗原进行ＳＤＳ－ＰＡＧＥ，以检测内分泌性眼病（ＥＯ）患者血清内的自身抗体。结果表明患者体内有一种针对甲状腺和眼球周围组织内分子量为６４０００抗原的交叉反应性抗体，其出现率较Ｇｒａｖｅｓ病患者和正常对照为高（Ｐ＜０．０５）。这种抗体不仅与甲状腺和眼球周围组织细胞膜上的６４０００抗原、也与甲状腺细胞浆内６４０００抗原发生反应。
The crude antigens of the cell
membrane and cytoplasm of the thyroid,eye muscles and periorbital connective tissues and
cellmembrane crude antigens of the skeletal muscle,and iiver were subjected to SDS-pAGE,then
immunoblotting with the sera of thepatients with endocrine ophthalmopathy(EO,n=27), patients
with Graves disease without ophthalmopathy(n= 20)and normalcontrols (n = 21 ). Results
indicated that there was a cross-reactive antibody which reacted with an antigen of 64 000
molecularweight in the thyroid and periorbital tissues. This antibody appeared more frequently
in the EO group than in the rest two groups,(P<0.05). The 64 000-molecular weight antigen not
only appeared in the cell membranes of the thyroid and periorbital tissues,but slso existed in
the thyroid cytoplasm,and to our knowledge this had not been reported in the literature. All
these results may beuseful for the elucidation of the pathogenesis of EO and its relations to the
thyroid abnormalities.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1994年第3期151-153,共3页
Chinese Journal of Endocrinology and Metabolism
关键词
WESTERN印迹
ELISA
内分泌性眼病
Ophthalmopathy,endocrine Cross-reactive antibody 64
000-molecular weight antigen
