摘要
以安哥拉山羊、建昌黑山羊、一代羊(F1)、二代羊(F2)、三代羊(F3)、二代与三代同质个体横交后代(H)为供试羊只,采用形态遗传标记、生化遗传标记和分子遗传标记确定安哥拉山羊改良建昌黑山羊生产马海毛的横交方案。结果:F2、F3 和H白色毛被分别为93 5%、100%和100%,毛被同质和基本同质个体分别为27 7%、70 2%和65 3%;成年羊产毛量随代数增加而增加,但F3 和H与F2,H 与F3 公、母羊分别对应比较,差异均不显著(P>0 05)。成年羊体重F1 和F2 增加,杂种优势明显,但F3 母羊则不显著(P>0 05),H还有下降(P>0 05)。平均基因杂合度和一致度安哥拉山羊、建昌黑山羊、F3、H分别为0 113 1 和0 886 9, 0 062 1 和0 937 9, 0 110 3 和0 889 7, 0 110 4和0 889 6。安哥拉山羊与F3 和H的遗传距离小,分别为0 003 9和0 004 0,遗传相似系数大,相应为0 996 1、0 996 0。群体内谱带相似系数,安哥拉山羊、F2、F3 和H分别为0 454 0,0 407 6,0 611 3 和0 610 9。且F3 和H均极显著大于安哥拉山羊(P<0 01)。综合研究表明,可在F2 和F3 代中选择理想同质个体进行横交。
Appearance, biochemical and molecular genetic markers were applied into the process of Mohair production using Angora goat gradebreed Jianchang black goat in order to establish crossbreeding program. Angora goat, Jianchang black goat and their generations F_1, F_2, F_3 and H were all studied in this research. The results of appearance genetic markers research showed that the individuals of white wool in F_2, F_3 and H generations were 93.5% ,100% and 100% respectively. The percentages of homogeneity and nearly homogeneity individuals in F_2 ,F_3 and H generations were 27.7%,70.2% and 65.3% respectively. The Mohaw yields of adult mals and females increased with the increased of generations ,but which not reached marked difference level between F_3 with H and F_2,H with F_3(P>0.05). The average adult body weights increased in F_1 and F_2 generation,but is not marked in F_3 generation(P>0.05), declined in H generation(P>0.05). The study of biochemical genetic marker demonstrated that average gene heterozygosity and gene consistency in Agora goat, Jianchang black goat, F_3 and H generations were 0.113 1 and 0.886 9, 0.062 1 and 0.937 9, 0.110 3 and 0.889 7, 0.110 4 and 0.889 6 respectively. The genetic distance is 0.003 9 and 0.004 0, the genetic similarity coefficient is 0.996 1 and 0.996 0 between Angora goats and F_3 and H generation. DNA fingerprinting technique as molecular genetic marker was also used in the research. The results revealed the similarity coefficient in different populations in Angora goats, F_2, F_3, and H generations were 0.454 0, 0.407 6, 0.611 3 and 0.610 9 respectively, which reached marked difference level between Angora goats with F_3 and H generations(P<0.01). The results of biochemical level and molecular level supported that cross breeding should be applied in F_2 and F_3 generation.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第6期550-554,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省"九五"重点攻关项目(1996002)
