摘要
目的:研究尿激酶型纤溶酶原激活因子(uPA)是否能诱导精子产生趋化性运动,从而探讨uPA在治疗男性不育中的可能作用机制。方法:采用精子聚集毛细管内的方法来检测精子趋化性。根据毛细管内自下而上uPA浓度梯度的方向将实验分为递增浓度A组、递减浓度B组和对照C组。A组毛细管内的趋化液以及精子培养皿内的处理液分别是不同浓度的uPA和Ham'sF-10;B组相反,分别为Ham'sF-10和uPA;C组毛细管和精子培养皿内的液体均为Ham'sF-10。然后检测在不同时间点不同组毛细管内聚集的精子密度。结果:①精子顺递增的uPA浓度梯度进行趋化运动。A组毛细管内液体对精子的聚集作用明显大于B组和C组(P<0.05)。②20IU/ml的uPA对精子趋化作用最强。③3组中的精子密度随着时间的延长趋于增加,但在20、30min2个时间点,3组的精子密度之间差异有显著性(P<0.05)。④uPA除了对精子有趋化作用外,还能增加精子活力,促进精子运动。结论:uPA在体外既能诱导精子产生趋化性运动,又能增加精子的活力,推测此为uPA治疗男性不育的作用机制之一。
Objective: To study the effects of the urokinase-type plasminogen activator (uPA) on the chemotactic responses of spermatozoa in vitro, and to explore the possible action mechanisms of uPA for male infertility. Methods: The chemotactic responses of spermatozoa were measured using spermatozoal accumulation in the capillary. According to the gradient directions of chemoattractant concentrations in the capillary, the recruits were divided into three groups: Group A (the ascending gradient of chemoattractant), Group B (the descending gradient of chemoattractant) and Group C (control). The chemoattractant in the capillary and the treating fluid in spermatozoal wells of Group A were uPA of different concentrations and Ham's F-10, respectively, while those of Group B were just opposite to Group A, and those of Group C were both Ham's F-10. Then the sperm densities in different capillaries were measured at different points of time. Results: ① Spermatozoa moved chemotactically following the concentrations of uPA. The accumulative action of spermatozoa in Group A was obviously stronger than in Groups B and C (P< 0.05). ② The effects of 20 IU/ml uPA on the chemotactic responses of spermatozoa were most significant. ③ The sperm densities in the three groups increased with time, significantly different at 20 min and 30 min ( P< 0.05). ④ uPA could increase sperm motility and promote sperm movement, as well as induce sperm chemotactic responses. Conclusion: uPA could induce sperm chemotactic responses and increase sperm motility, which is presumably one of the action mechanisms of uPA on male infertility.
出处
《中华男科学杂志》
CAS
CSCD
2005年第6期409-412,418,共5页
National Journal of Andrology
基金
国家"十五"攻关计划课题(2004BA720A33-1)
关键词
尿激酶型纤溶酶原激活因子
精子
趋化性运动
urokinase-type plasminogen activator
spermatozoa
chemotactic response
