摘要
目的建立念珠菌定植的阴道感染小鼠模型。方法先用林可霉素溶液(浓度为100mg/ml)冲洗小鼠阴道,再将念珠菌接种到小鼠阴道内。结果模型组小鼠阴道内主要细菌数量乳杆菌显著下降(P<0.01),出现阴道红肿、分泌物多呈块状和阴道黏膜充血等念珠菌阴道炎的典型症状。结论通过抗生素处理后,再在小鼠阴道内接种念珠菌,念珠菌能在小鼠阴道内定植,即建立了念珠菌性阴道炎的小鼠模型。
Objective To establish a mouse model with pathological Candida albicans in the vagina.Methods Six-eight week old mice were vaginal washed with antibiotic,then inoculated intra-vaginally with 20 μl of a suspension of Candida albicans to make mouse vaginal Candida imbalance model.Results The amounts of Lactobacillus in the vaginal of model mouse decreased significantly (P<0.01).The typical symptoms such as vaginal swell,clumpish secretion and congestive vaginal mucous membrane was appeared in the vagina of model rats,white blood cell and Candida albicans could be found when the vaginal secretion examined by microscopy.Conclusions A persistent vaginal colonization with pathological Candida albicans induced by administration of lincomicin was established in mouse.This animal model was proposed for the study of Candida albicans in future.
出处
《中国微生态学杂志》
CAS
CSCD
2005年第3期168-169,共2页
Chinese Journal of Microecology
