摘要
目的:研究补阳还五汤含药血清对人内皮型一氧化氮合酶(eNOS)启动子活性的影响。方法:基因亚克隆方法构建pGL2 eNOS和pEGFP- 1 -eNOS质粒载体;应用jetPEITM转染内皮细胞ECV304,分别用HTS7000生化分析仪和荧光显微镜观察报告基因的表达。结果:含补阳还五汤及黄芪的血清均能增强 pEGFP -1- eNOS转染的内皮细胞的绿色荧光蛋白的表达,并能增强转染 pGL2- eNOS荧光素酶的表达。结论:补阳还五汤和黄芪可能通过增强内皮型eNOS启动子的活性、延缓内皮功能障碍的发生以防治动脉粥样硬化。
Objective: to study the effects of buyang huanwu decoction on the promotor activity of endothelial nitric oxide synthase (eNOS). Methods: pGL2-eNOS and pEGFP-1-eNOS were constructed by subclone method. The activity of pGL2-eNOS was measured by HTS 7000 Bio assay reader and the expression of pEGFP-1-eNOS was observed by fluorescence microscope. Results: the activity of both pGL2-eNOS and pEGFP-1-eNOS increased after treated by buyang huanwu decoction containing serum and astragalus membranaceus containing serum in ECV304 cells. Conclusion: buyang huanwu decoction increased the activity of the eNOS promotor. It must be the mechanism of buyang huanwu decoction in preventing the endothelial dysfunction.
出处
《山东中医杂志》
2005年第3期162-165,共4页
Shandong Journal of Traditional Chinese Medicine
基金
广东省中医药管理局科研课题(1040088)
