摘要
目的 从蛋白和DNA两个水平初步检测成都地区结外鼻型NK/T细胞淋巴瘤中L MP1的表达,并探讨与预后的关系。方法 应用免疫组化和PCR技术检测6 7例结外鼻型NK/T细胞淋巴瘤L MP1的表达,并应用Kaplan- Meier曲线分别比较L MP1蛋白和L MP1DNA阳性表达组与阴性表达组的生存率。结果 L MP1蛋白阳性表达10例(14 .93% ) ,L MP1DNA阳性表达5 6例(83.5 8% ) ,L MP1总检出率83.5 8%。L MP1蛋白(P=0 .6 78)和L MP1DNA (P=0 .94 3)表达均与预后无明显关系。结论 L MP1与成都地区结外鼻型NK/T细胞淋巴瘤关系密切;结外鼻型NK/T细胞淋巴瘤中L MP1蛋白水平和DNA水平表达不一致;L MP1的表达与预后无明显关系。
Objective To investigate the expression and prognostic significance of Epstein-Barr virus latent membrane protein 1 in extranodal nasal type NK/T-cell lymphoma in the Chengdu area. Methods The expression of latent membrane protein-1(LMP1) was detected by immunohistochemistry (IHC) and DNA-PCR in 67 cases of extranodal nasal type NK/T-cell lymphoma, and the differences in survival rate between positive and negative expression groups of LMP1-protien and LMP1-DNA were analyzed respectively. Results Ten(14.93%)cases were positive at LMP1-protein level, and fifty-six(83.58%)were positive at LMP1-DNA level. The total expression rate of LMP1 was 83.58%. No statistically significant difference was observed between the expression of LMP1 and prognosis(P=0.678) and between the expression of LMP1-DNA and prognosis (P=0.943). Conclusion LMP1 was shown to be closely associated with extranodal nasal type NK/T-cell lymphoma in Chengdu. The expression rate of LMP1 at protein level was different from that at DNA level. No relationship was found between the prognosis and the LMP1 expression in extranodal nasal type NK/T-cell lymphoma.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期338-340,共3页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金 (批准号 3 0 470 747)资助
