摘要
目的探讨颅脑外伤后细胞凋亡与Fas抗原表达的变化。为阐明损伤后神经元丢失的多种途径提供实验依据。方法建立大鼠自由落体脑创伤模型,采用免疫组化技术及细胞透射电镜观察形态学的方法,对伤后挫伤灶旁局部脑组织细胞凋亡和Fas抗原表达进行观察。结果在挫伤灶旁观察到细胞凋亡的发生,神经细胞呈现较为典型的凋亡超微结构变化,Fas蛋白表达在24h达到高峰,随后呈下降趋势。结论颅脑外伤后在局部组织可见细胞凋亡的发生,Fas蛋白表达参与了外伤后神经细胞凋亡的过程。
Objective To evaluate the change in Fas antigen expression and apoptosis of neurons, to provide an experimental evidence of loss of neurons in craniocerebral injury, and to provide experimental evidence for clarifying multi - approach of the lost neuron after damage. Methods Brain impact injury was reproduced in SD rat with a free falling impacting device. Using immunohistochemistry method the Fas protein expression was assessed and apoptotic cells were detected with electron microscopy. Results Apoptotic cells were found near the contused area, and Fas - positive cells appeared around the injured and hippocampus areas at about 4 hours, peaked at 24 hours after injury and then reduced in number. Conclusion Apoptosis and necrosis are two forms of cell death in brain tissue following experimental brain contusion. Moreover, the results imply that the Fas - FasL pathway plays a pivotal role in the pathophysiology of post - traumatic neuronal apoptosis.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2005年第5期286-288,i002,共4页
Chinese Critical Care Medicine
基金
上海市属高校自然科学基金资助项目(01B01)
