摘要
根据ｓＩＬ－６Ｒ的结构特点及其功能分析，利用ＰＣＲ技术选择性地扩增两个不同长度的ＩＬ－６ＲｃＤＮＡ胞外片段。经ＤＮＡ测序和Ｓｏｕｔｈｅｒｎｂｌｏｔ证实后，定向插入原核表达载体ｐＥＴ－３ａ中，获得了中、高效表达。
ccording to the structurc and
function of human IL-6 recceptor,we amplified two genefragments of
IL-6R cDNA specifically using PCR technique. The two PCR products
were iden-tified by southern blot and DNA sequeneing, then were
inserted into expression plasmid pET-3a. Both of them,which encode
extracellular peptide chains of IL-6R,reached to middle andhigh
expression separtely in E. coli.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1994年第1期25-27,共3页
Chinese Journal of Immunology
