摘要
目的研究三氧化二砷(As2O3)对培养的人视网膜色素上皮(humanretinalpigmentepithelium,HRPE)细胞的不良反应。方法培养的HRPE细胞分为血清组和无血清组,用As2O3处理HRPE细胞48h,倒置显微镜下观察细胞形态,四唑盐(MTT)比色法检测As2O3对HRPE细胞生长的影响,流式细胞仪检测As2O3处理HRPE细胞后的细胞周期变化。结果镜下观察,血清组细胞药物处理后在25μmol/LAs2O3作用下出现典型凋亡细胞形态。无血清组细胞部分细胞体积增大。MTT结果显示血清组细胞3.12μmol/LAs2O3时即表现出抑制作用,无血清组细胞6.25μmol/L时表现出抑制作用,流式细胞仪检测示两组细胞药物处理后细胞周期变化无区别。结论As2O3对不同生长状态下的HRPE细胞不良反应不同,可为临床玻璃体腔内用药防治增生性玻璃体视网膜病变(proliferativevitreoretinopathy,PVR)提供新的药物干预和实验数据。
Objective To study the effects of arsenic trioxide(As 2O 3) on cultured human retinal pigment epithelial (HRPE) cells. Methods HRPE cells cultured were divided into two groups: serum group and free serum group.They were treated with arsenic trioxide for 48 hours. Cytomorphology was investigated under microscope, cell proliferating activity was assessed using the MTT colorimetric assay, while cell cycle status was determined using flow cytometry. Results Morphologically, we can see that serum group HRPE cells treated with arsenic trioxide presented apoptosis feature when exposure to 25μmol/L arsenic trioxide. While in free serum group HRPE cells showed the volume of some cells was enlarged. The results of MTT revealed 3.12μmol/L arsenic trioxide inhibited the proliferation of HRPE cells in a concentration-dependent manner, free serum group showed inhibition at 6.25μmol/L. Flow cytometry analysis indicated changes of cell cycle distribution in HRPE cell were not different. Conclusion Arsenic trioxide can inhibit the proliferation of HRPE cells.The toxicity in serum group was more marked than in free serum group.The study provided a new medication and experimental data for prevention of PVR clinically.
出处
《医药论坛杂志》
2005年第7期26-27,54,共3页
Journal of Medical Forum
