摘要
目的 为探讨血红素氧合酶 1基因启动子双核苷酸重复序列的微卫星多态性与冠状动脉支架术后再狭窄的关系。方法 回顾分析 118名冠状动脉支架置入术患者 ,随访冠状动脉造影分为支架内再狭窄组和无再狭窄组 ;提取患者外周血DNA ,经聚合酶链反应扩增微卫星序列后采用Spreadex凝胶电泳进行基因分型。结果 入选患者中有 5 8% (6 8/ 118)发生支架内再狭窄 ,其中携带双核苷酸重复 <2 5次等位基因患者的再狭窄率为 4 7.5 % ,携带两条双核苷酸重复均≥ 2 5次等位基因患者的再狭窄率为 6 8.4 % (P <0 .0 5 )。经多元回归分析校正冠心病危险因素及介入治疗的相关影响因素后 ,两组的再狭窄率仍有统计学差异 (OR为 0 .4 0 6 ,95 %CI为 0 .185～ 0 .891,P<0 .0 5 )。结论 血红素氧合酶 1基因启动子微卫星多态性与再狭窄相关 ,对调控血红素氧合酶
Aim To determine if an association exists between restenosis after percutaneous coronary intervention and heme oxygenase-1 (HO-1). A dinucleotide (guanosine thymidine, GT) repeat in the promotor region of the HO-1 gene shows a polymorphism that modulates the level of gene transcription. Methods This retrospective study included 118 patients of the Han nationality (92 men; mean age 62.3 years, standard deviation 9.8) who underwent successful stent implantation. Patency follow-up was evaluated using angiography. The length of GT repeat was confirmed using polymerase chain reaction (PCR) amplification and electrophoresis. Selected samples were sequenced by means of Sanger's method. The association of patency and the length of GT repeat in the HO-1 gene promotor was assessed in univariate and multivariate analyses. Results In-stent restenosis was found in 68 (58%)out of 118 patients. Patients with short (<25GT) dinucleotide repeats in the HO-1 gene promotor on either allele had restenosis significantly less often than patients without short dinucleatide repeat (47.5% vs 68.4%,P< 0.05 ). Multivariate analysis revealed a significantly similar result after controlling certain possible confounding factors (odd ratio 0.406, 95%CI 0.185 ～0.891, P<0.05). Conclusions In this patient population, short GT repeat alleles of the HO-1 gene promotor polymorphism were associated with reduced post-PCI restenosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第1期91-93,共3页
Chinese Journal of Arteriosclerosis
