摘要
利用397条SSR引物对D62A和红米水稻红宝石B的杂交F2代不育可育分离群体及双亲的12条染色体进行筛选,定位细胞质雄性不育恢复基因,发现在第7染色体上找到与主效恢复基因紧密连锁的分子标记RM182,遗传距离是7 4cM。
Bulked segregant analysis (BSA) of a F_2 population derived from D62A/ Ruby B was used to map the nuclear fertility restorer gene for wild abortive (WA) cytoplasmic male sterility. Three hundred and ninety-seven microsatellite primer pairs,which distributed on 12 chromosomes,were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM182 located on chromosome 7 produced polymorphic products. The nuclear fertility restorer gene for WA cytoplasmic male sterility was mapped on chromosome 7, 7.4 cM from RM182.
出处
《西南农业学报》
CSCD
2005年第2期122-124,共3页
Southwest China Journal of Agricultural Sciences
基金
四川省科技厅青年基金资助
科技部国际合作项目
财政部亚太地区合作项目资助
