摘要
番木瓜环斑病毒（ＰＲＶ）提纯的研究高文通（黄埔动植物检疫局，广州５１０７００）高乔婉，范怀忠（华南农业大学植保系，广州５１０６４２）关键词番木瓜环斑病毒，病毒提纯四十年代末，美国首次报道的番木瓜环斑病（ＰａｐｅｙａＲｉｎｇｓｐｏｔＶｉｒｕｓ，ＰＲＶ）...
very best choice of several rnain affecfing factore on the crude purification of PRV was reporteddetailediy in this paper.The advanee purification method of PRV with the Cs_2SO_4 continuous densitygradient supercentrifusation for about 3 hours with 30% sugar cushion was tested for the first time.Asimple procedure of one cycle of different speed centrifugution and one cycle of Cs_2SO_4 continuous density gradient supercentrifugation was designed at last.The virus preparation showed a typical ultravio-let absorption curve for nucleoproteiris,The yield of virus was 2.56mg/100g of infected squashleaves.
出处
《中国病毒学》
CAS
CSCD
1994年第2期180-182,共3页
Virologica Sinica
基金
国家自然科学基金
关键词
提纯
番木瓜环斑病
病毒
Papaya Ringrpot Viras,Purification of virus
