摘要
应用淋巴细胞杂交瘤技术生产出针对大肠埃希氏菌K88粘附素a、b、c和d4种抗原因子特异的单克隆抗体(MCA)12株。经酶联免疫吸附试验(ELISA)、间接免疫荧光试验(HF)和直接凝集试验(DA)证明:K88a因子特异MCA有4株,与所试的33株K88菌株都产生阳性反应;K88b、K88c和K88d因子特异MCA各1株,分别与所试的3株K88ab菌株、22株K88ac菌株和6株K88ad菌株都产生阳性反应。用MCA对K88粘附素抗原结构进行分析表明:在K88ab、K88ac和K88ad三种血清型中,同一种血清型的菌株至少能表达一个共同的型特异抗原决定簇;K88ad菌株和K88ac菌株都能稳定地表达一个K88ab菌株不具有的共同的抗原决定簇;某些抗原决定簇仅存在于同一种K88血清型的部分菌株中。
A panel of twelve hybridoma cell lines, secreting specific antibodies to K88 adhesin antigens of enterotoxigenic Escherichia coli (ETEC) were established from eight separate fusions between mouse myeloma cell line Sp 2/0-Ag-14 and spleen cells from mice immunized with purified K88 antigens. Among the 12 monoclonal antibodies (MCA), K-A, K-35, K-11, and K-15 were K88a specific and reacted with all K88 adhesin bearing Escherichia coli strains tested, whatever K88ab, K88ac or K88ad they might be, as shown either in enzyme-linked immunosorbent assay (ELISA) or in direct agglutination test, whereas K32, K-4, and K-3 were sprcific for G88ab, K88ac, and K88ad respectively.
The antigen pattens of 33 K88 bearing Escherichia coli strains covering 3 serotypes of K88ab, K88ac, and K88ad were analysed by the use of these MCAs. The preliminary results showed that all Escherichia strins with the same serotype of K88 antigen shared at least one common typespecific antigenic determinant, that K88ad and K88ac strains enjoyed one common antigenic determinent that did not exist on K88ab strains, and that there were a few K88 antigenic determinents that appeared only on limited Escherichia coli strains of the same K88 serotype.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第5期348-353,共6页
Acta Microbiologica Sinica
