摘要
建立反相高效液相色谱法分离和测定迷迭香中鼠尾草酸的含量。以Hil sil C18反相键合硅胶色谱柱(250mm×4.6mm,5μm),乙腈+甲醇(85:15)为流动相,流速1.0ml/min,检测波长为210nm,外标法定量。鼠尾草酸在20～200μg/ml范围有良好的线性关系,r=0.9998,回收率为98.6%。本方法准确、可靠、简便,可用于迷迭香中鼠尾草酸的含量测定。
To establish a HPLC method for the separation and determination of Carnosic acid in Rosemarinus officimalis L, the Pheromenex C18 reverse Phase column (250mm×4.6mm, 5μm) and acetonitrile-methanol as mobile phase were used with flow rate 1.0ml/min detecting spectrum 210 nm and quantitating value by external standard method. The standard curve of linear relationship was of 20～200μg/ml and r=0.9998.The average recovery was 98.6%. The established method was accurate, sensitive, simple and reliable for the determination of Carnosic acid in Rosemarinus officinalis L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第4期203-205,共3页
Food Science
基金
国家火炬计划项目(2002EB021188)
