摘要
目的:研究慢传输便秘(STC)大鼠的结肠肌电变化,并从神经病理学的角度初步探讨其发生机制. 方法:应用复方苯乙哌啶建立大鼠慢传输型便秘模型,采用电生理技术进行结肠肌电检测. 利用铺片技术制作结肠肌间神经丛标本,采用免疫组织化学法在铺片上显示大鼠结肠肌间神经丛内VIP能神经和SP能神经的分布及形态学改变. 结果:模型组大鼠结肠慢波出现双向改变,部分大鼠结肠慢波频率明显减慢[ (5. 55±1. 20)次/minvs对照组(13. 20±1. 09)次/min,P<0. 01],振幅增加[ ( 0. 43±0. 05 )mVvs对照组( 0. 19±0. 02)mV,P<0. 01],波形仍表现为不规则的近似正弦波样曲线;部分大鼠结肠慢波频率出现快速性改变[ ( 30. 85±3. 86)次/minvs对照组(13. 20±1. 09)次/min,P<0. 01 ],振幅强弱不等[ (0. 21±0. 03)mVvs对照组(0. 19±0. 02)mV,P<0. 01],波形较不稳定,且出现基线位移. 模型组大鼠结肠慢波频率和振幅变异系数分别为22. 2%, 12. 5%和11. 8%,15. 1%,均显著高于对照组大鼠(8. 3%和8. 4%, P<0. 01).免疫组化染色发现模型大鼠VIP阳性神经节及节间束细小,节内神经元数量明显减少,神经束内神经纤维分布稀疏,阳性神经元胞体少见,其平均面密度值为19. 35±1. 13,显著低于对照组(23. 08±1. 82,P<0. 01 ). SP阳性神经节明显增?
AIM: To study the changes of colonic myoelectric activity of rats with slow transit constipation and to explore the pathogenesis by investigating the changes of VIP and SP nerves. METHODS: Thirty-two healthy Wistar rats were randomly divided into control group and model group with slow transit constipation. After 120 d, the colonic myoelectric activity was recorded and the time of colonic transit was measured. The VIP nerves and SP nerves were studied in colonic myenteric plexuses of the rats with histochemistry and immunohistochemistry. RESULTS: In some model rats, the frequency of colonic slow wave decreased [(5.55±1.20) beat/min vs (13.20±1.09) beat/min, P <0.01] and the amplitude increased [(0.43±0.05) mV vs (0.19±0.02) mV, P <0.01] compared with those in the normal rats, and the slow wave form was irregular. In other model rats, the frequency of colonic slow wave increased [(30.85±3.86) beat/min vs (13.20±1.09) beat/min, P < 0.01] and the amplitude was variable [(0.21±0.03) mV vs (0.19± 0.02) mV, P <0.01] and the slow wave form was also irregular. In the colonic myenteric plexuses of rats with slow transit constipation, the VIP immunoreactive positive nerves and cells were significantly lessened (with surface density 19.35±1.13) than that in the control group (23.08± 1.82, P <0.01). The SP immunoreactive positive ganglions and fascicles were significantly larger and SP immunoreactive positive cells increased (with surface density 18.14±1.18) compared with that in normal rats (15.45± 1.05 , P <0.01). CONCLUSION: Abnormality of colonic myoelectric activity may be one of the mechanisms of slow transit constipation. Dysfunction of colonic transition is associated with the pathological changes of VIP and SP nerves.
出处
《第四军医大学学报》
北大核心
2005年第7期603-606,共4页
Journal of the Fourth Military Medical University
基金
杨森科学研究基金(JRC14)
