摘要
基于甲基紫 6B与脱氧核糖核酸在酸性条件下共振光散射的增强效应 ,建立了测定DNA的共振光散射法。在 pH值为 2 .0～ 3.0的三羟甲基氨基甲烷 -盐酸缓冲溶液中 ,甲基紫 6B与fsDNA、ctDNA分子作用后共振光散射增强 ,其强度增加值与DNA的浓度呈线性关系 ,线性范围分别为 0～ 1.0、0～ 1.5mg/L ,相关系数分别为 0 .9993、0 .9997,检出限分别为 15 .3、12 .2 μg/L ,用于DNA合成样品的测定 ,测定结果的精密度、准确度较高。
Resonance light-scattering (RLS) characteristics of interaction between methyl violet 6B and deoxyribonucleic acid (DNA) was studied in the medium with pH 2.36. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of the DNA (fsDNA,ctDNA) at nanogram level based on RLS measurement. The linear range of the fsDNA is 0～1.0 mg/L and the ctDNA is 0～1.5 mg/L with the correlation coefficient of 0.999 3 and 0.999 7 respectively. The detection limit is 15.3 μg/L and 12.2 μg/L. The method has been applied to the determination of DNA in mixed samples with high accuracy and precision.
出处
《化学分析计量》
CAS
2004年第5期12-14,共3页
Chemical Analysis And Meterage
基金
湖南省自然科学基金资助项目 (0 0JJY2 0 84)
湖南省教育厅资助项目 (0 3C61 1 )
