摘要
[目的] 探讨中药肠道泡腾栓剂治疗溃疡性结肠炎(UC)的免疫学机制。[方法] 实验分设正常对照、模型对照、空白泡腾栓、清肠泡腾栓、清肠栓、柳氮磺胺吡啶(SASP)栓共6 组,ELLESA法检测血清、结肠上清肿瘤坏死因子- α(TNF -α)、白细胞介素13(IL- 13)。[结果] 模型组血清、结肠上清TNF- α较正常对照组升高,IL- 13 分别轻度降低和升高;清肠泡腾栓组明显降低结肠上清TNF -α含量,与模型对照组比较差异有统计学意义(P <0. 05)。[结论] ①模型组TNF -α、IL -13较正常组升高或降低,提示UC免疫学发病机制的可能性;②清肠泡腾栓组下调UC模型大鼠TNF -α,对IL- 13的调控作用则不甚明显,提示清肠泡腾栓可能在抑制促炎细胞因子方面具有更为确切的优势。
Objective] To explore the effect of Qingchang effervescence suppository(QES) on ulcerative colonitis(UC) and demonstrate its immune therapeutic mechanism. [Methods] All rats were divided into six groups as follows: normal control group, model control group, blank effervescence suppository group(BES), QES group, Qingchang suppository group(QS) and SASP group. After 2-week experimental period, the rats were killed to assay the TNF-alpha and IL-13 in serum and colon mucosa. [Results] Compared with the normal control group, TNF-alpha content in the serum and colon mucosa of model group was increased, while the IL-13 in the serum was increased and that in the colon mucosa decreased. Compared with the model control group, QES reduced the TNF-alpha content in the colon mucosa of UC ( P <0.05). [Conclusion] QES could reduce the TNF-alpha of UC, while the effect on IL-13 was not very obvious, which showed more affirmative predominance of QES on inhibiting phlogogenic cytokines.
出处
《中国中西医结合消化杂志》
CAS
2005年第2期94-96,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
上海市科委2002年博士发展基金资助项目
关键词
清肠泡腾栓
溃疡性结肠炎
肿瘤坏死因子-Α
白细胞介素-13
Qingchang effervescence suppository(QES)
ulcerative colonitis(UC)
TNF-alpha
interleukin-13
experimental study
