摘要
本文研究结果表明，亚硫酸氢钠（二氧化硫）能够引起人血淋巴细胞姊妹染色单体互换（ＳＣＥ）和微核（ＭＮ）率的增加，可使淋巴细胞有丝分裂周期延迟及细胞分裂指数下降，且这些作用有显著的剂量效应关系。结果指出，亚硫酸氢钠在低浓度下仅引起细胞染色单体型畸变，在高浓度下既可引起染色单体型畸变，又可引起染色体型畸变。结果还指出，亚硫酸氢钠对染色体畸变（ＣＡ）和ＭＮ的诱发效应有明显的个体差异。硫酸钠未能引起上述细胞遗传学效应。这些结果表明，二氧化硫确是人血淋巴细胞染色体断裂剂和基因毒性因子。
The frequencies of chromosomal aberrations (CA),sister chromatid exchanges (SCE) and micronuclei(MN) in human blood lymphocytes exposed to sodium bisulfite (sulfur dioxide) at varies concentrations ranging from 5×10￣(-5) to 2×10￣(-3)mol/L in vitro were studied. It was shown that sodium bisulfi (NaHSO3 and Na2SO3,1:3 mol/L)caused an increase in SCE and MN of human blood lymphocytes in a dose-dependent manner, and also induced mitotic delays and decreased mitotic index of the lymphocytes.For CA, our results indicated that sodium bisulfite induced an increase of chromatid-type aberrations of the lymphocytes from three of four donors in a dose-dependent manner. The chemical at low concentrations induced chromatidtype aberrations but not chromosome-type aberrations; at high concentrations inducedboth chromatid and chromosome-type aberrations of lymphocytes. No cytogenetic effects of sodium bisulfate on the human blood lymphocytes were observed in these assays. The results have confirmed that sulfur dioxide is a clastogenic and genotoxic agent.
关键词
亚硫酸氢钠
微核
染色体畸变
SCE
Sulfur dioxide, Sodium bisulfite, Micronuclei, Chromosomal aberrations,Sister chromatid exchanges,Lymphocytes
