摘要
目的探讨肌成纤维细胞对糖尿病肾病发生发展的意义。方法链脲佐菌素诱导SD大鼠糖尿病模型,病程1、2、4、8周,利用组织学检查观察肾组织结构的改变,免疫组化法测定肾组织αSMA和型胶原(C-)的表达,同时用双抗体夹心法检测肾皮质αSMA含量。结果糖尿病大鼠肾皮质αSMA含量在各观察时点均高于正常对照组(P<0.05)。同时,8周内糖尿病大鼠肾组织C-的染色始终较正常对照组强(P<0.05),且与αSMA表达呈正相关。结论糖尿病早期大鼠肾脏αSMA表达增高,预示肌成纤维细胞的形成,它可能参与C-等细胞外基质的堆积。
Objective: To investigate the expression of α smooth muscle actin(αSMA),a marker of myofibroblast in kidney of diabetic rats and its role in pathogenesis of diabetic nephropathy. Methods: Diabetic disease model was made on 24 Sprague-Dauley(SD) rats by an single intraperitoneal injection of streptozotocin (70 mg/kg) and 24 health SD rats served as controls.Six diabetic rats and six control rats were sacrificed at 1,2,4,8 weeks after injection of streptozotocin.At each time-point,the renal morphological changes were examined by histopathology.αSMA and collagen-Ⅳ (C-IV) expressions were studied by immunohistochemistry (SABC).The content of αSMA in renal cortex was detected by enzyme-linked immunosorbent assay(ELISA). Results: At all time-points,renal content ofαSMA increased in diabetic rats. Compared with controls,diabetic rats had a steady increased accumulation of C-IV.Expression of C-IV was positively correlated with that of α SMA. Conclusion: At early stage of diabetes,increased α SMA in kidney suggests the formation of myofibroblast,which may be involved in accumulation of C-IV and contributed to diabetic nephropathy. [
出处
《浙江大学学报（医学版）》
CAS
CSCD
2005年第2期152-156,共5页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省自然科学基金资助项目(300506)
浙江省科技厅科研基金资助项目(2002c330)
