摘要
目的 :建立以高效液相色谱法测定莪术中吉马酮含量的方法。方法 :色谱柱为C18,流动相为乙腈 -水 (梯度洗脱 ) ,流速为1 .0ml/min ,检测波长为214nm。结果 :吉马酮检测浓度在0. 95～285μg/ml范围内线性关系良好 (r=0 .9999) ,平均回收率为97.89 % (RSD=2.52 % ,n=5)。结论 :本方法简便、可靠 ,可用于莪术药材的含量测定和质量控制。
OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285μg/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第6期461-462,共2页
China Pharmacy
关键词
效液相色谱法
莪术
吉马酮
含量测定
HPLC
Rhizoma curcuma
Germacrone
Content determination
