摘要
以三倍体无籽西瓜顶芽为外植体，在ＭＳ＋ＢＡ３ｍｇ／Ｌ琼脂培养基上诱导丛芽发生，于ＭＳ＋ＢＡ２ｍｇ／Ｌ＋ＩＡＡ１ｍｇ／Ｌ培养基上继代培养，周期２０～３０天。平均繁殖系数，前１０代为８左右，１～２０代为５左右，１个芽在半年内可形成８～１０万株苗。丛芽最高代次为３０代，芽的分化与生长正常。将伸长芽嫁接在瓠瓜上，成活率达９０％以上，移栽成活率可达９５％以上。
Bud explants with cotyledons cut off from sterilized watermelon seedlings developed fromtriploid seeds were cultured on MS medium supplemented with 3 mg/L BA to induce bud clustersand subcultured on MS medium supplemented with 2 mg/L BA and 1 mg/L IAA.The culture periodlasted 20 to 30 days.The propagation first 20 generations.A bud explant regenerated 80,000 to100,000 plantlets.The clones of proliferated meristems have been mairitained for 30 clonegenerations through subculture and the buds have all grown and developed normally. When graftedon the stocks of Lagenaria siceria var.clavata,most of the elongated buds of watermelon survived witha survival rate of 90% of the grafted plants survived.
出处
《西南农业大学学报（自然科学版）》
CSCD
1994年第6期540-542,共3页
Journal of Southwest Agricultural University
关键词
西瓜
组织培养
培养基
无籽西瓜
watermelon
tissue cultures
clutre media/seedless watermelon
