摘要
在体外通过Ｆｅｎｔｏｎ反应系统生成羟自由基（ＯＨ），用自由基捕捉剂ＤＭＰＯ捕捉生成的ＯＨ，形成保留ＯＨ性质的ＤＭＰＯ－ＯＨ自旋加合物，用电子自旋共振仪（ＥＳＲ）检测ＯＨ的生成量。研究发现，在Ｆｅｎｔｏｎ反应系统中加入不同浓度的氯酯醒，ＥＳＲ检测到的自旋加合物信号明显减弱。实验证实，氯酯醒具有羟自由基清除作用。
The fenton reaction system generates OH'free radical which can be trapped by 5, 5-dimethy-1-pyrroline-N-oxide(MPO) thus yielding a measurable signal by clectron spin resonance(ESR) spectroscopy. We found that the signal of DMPO-OH'spin adduct detected using ESR remarkedly decreased when the meclophenoxate of different concentrations proved to be an OH'free radical scavenger in the fenton reaction system. On the basis of the experimental results, the OH'free radical scavenger property of meclophenoxate can be regarded as firmly established.
出处
《南通医学院学报》
1994年第2期176-177,共2页
ACTA Academiae Medicinae Nantong
