摘要
以杂交长白猪为动物模型，采用节段小肠二步移植法作同种异体移植。研究分两个阶段进行，第一阶段为观察雷公藤多甙抑制急性排斥的作用。在同窝猪无免疫抑制剂组（Ⅰ组）、大剂量环孢素Ａ（ＣｓＡ）组（Ⅱ组）、小剂量ＣｓＡ组（Ⅲ组）、雷公藤加小剂量ＣｓＡ组（Ⅳ组）中，Ⅰ、Ⅲ组均发生急性排斥，但Ⅱ组中有２只猪因严重感染死亡。第二阶段为观察雷公藤抑制慢性排斥的作用，将Ⅱ、Ⅳ组存活１００ｄ以上的猪分为停用免疫抑制剂（２只）、减量雷公藤（２只）、常量雷公藤（３只）三组，结果示常量组存活３５６～５４７ｄ，无慢性排斥发生，而其余４只猪中有３只发生了慢性排斥。实验的结果说明，雷公藤对异体小肠移植的急、慢性排斥有明确的抑制作用。
This study was proceeded in two stages in order to evaluate the effect of TW on acute andchronic rejection of small intestinal allografts separately. In the first stage, the pigs with allografts weredivided into 4 groups according to the different immunosuppresive regimens. Acute rejection of allograftoccured in animals not treated with immunosuperesive agent or treated with low dose of cyclosporine A(CsA). No rejection developed in the animals treated with high dose CsA or low dose CsA TW, Sevenpigs survived more than 1 00 days after transplantation were selected for the study of chronic rejection。Chronic rejcetion of allograft developed in animals when the immunosupperesive treatment was discontin-ued or the dosage of TW was reduced. No evidence of chronic rejection was found in 3 pigs with continuedadministration of TW 2mg/(kg·d). These 3 pigs were sacrificed due to trauma or volvulus of host intes-tine at 356,397 or 547 days respectively after transplantation. No abnormalities were found on organs andallografts at autopsy
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1994年第4期255-260,共6页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
雷公藤
多甙
排斥反应
小肠移植
Tripterygium wilfordii
Reject reaction
Allograft
Pigs
Small intestine transplantation
