摘要
以甘蓝型单低和双低油菜为材料，对原生质体培养及植株再生进行了研究。结果表明：低温预处理无菌苗可提高原生质体活力。不同下胚轴部位对原生质体的得率及分裂频率均有影响。用琼脂糖包埋法，在不含ＮＨ４ＮＯ３及ＫＮＯ３，而加有丝氨酸和谷氨酰胺的Ｋｍ８Ｐ培养基上培养原生质体获得了高频率再生愈伤组织。在Ｍ＋ＢＡ１ｍｇ／Ｌ分化培养基上两周后得到芽的分化，转至ＭＳ＋ＮＡＡ０．１ｍｇ／Ｌ＋生根粉０．１ｍｇ／Ｌ的培养基上诱导生根，得到生长良好的完整植株。
The results showed that the viability of protoplasts were promoted by low temperature pretreatment (4℃, 1h) of the seedlings.The yield and the division frequency of protoplasts were influenced by different part of hypocotyl.The high regeneration frequency were obtained when the proplasts were embedded in agarose and cultured in Km8P medium added glutamine and serine without NH4NO3 and KNO3.Shoot regeneration occurred when the protoplast-derived calli were transferred onto M+ BA 1mg/L differentiation medium.Individual shoot was rooted on MS+NAA 0.1 mg/L +rooting powder 0.1 mg/L medium,whole plants were gotton and grown well.
出处
《吉林农业大学学报》
CAS
CSCD
1994年第4期77-81,86,共6页
Journal of Jilin Agricultural University
基金
上海市科委资助项目
关键词
油菜
甘蓝型
单双低油菜
原生质体培养
植株再生
Single low and double low brassoca napus
Protoplast culture
Protoplast embedded in agarose
Plant regeneration
