摘要
本文研究了ｍｅｓｏ－四－（４－甲氧苯基）卟啉与Ｃｕ（Ⅱ）、Ｚｎ（Ⅱ）的共显色条件和导数光谱条件，提出了用偏最小二乘法（ＰＬＳ）解析导数光谱进行Ｃｕ、Ｚｎ同时测定的计算分析方法。在ｐＨ７．０５的介质中，有表面活性剂Ｔｗｅｅｎ－８０存在时，于沸水浴内加热１６ｍｉｎ，Ｃｕ（Ⅱ）、Ｚｎ（Ⅱ）均能显色完全。采用四阶导数光谱进行测量，其摩尔响应系数（峰－峰法）分别达９．１４×１０￣６和１．５３×１０￣７。该法用于Ｃｕ：Ｚｎ为０．０３：０．５０～０．４０：０．０２（μｇ／２５ｍＬ）的合成样品测定，相对误差在±５％以内；用于人体血清中Ｃｕ、Ｚｎ及Ｃｕ／Ｚｎ的测定，结果与原子吸收光谱法相吻合。
The common color reaction conditions and thederivative spectral conditions for meso-tetrakis-(4-methoxyphenyl)-porphyrin (TMOPP)with Cu(Ⅱ)and Zn (Ⅱ)have been investigated.A derivativespectrophotometric partial least so uares(PLS)methodfor the simultaneous determination of copper,zinc andtheir ratio has been developed.Heated for 16min in aboiling water-bath and in the neutral medium of pH 7.05 and in the presence of Tween-80 , the color reactionof TMOPP-Cu ,- Zn can proceed quantitatively. Their4th-derivative molar responsive coefficients(peak to-peak)are 9.14×10￣6 and 1. 53×10￣7,respectively. Theproposed method has been applied to the determinationof Cu,Zn and Cu/Zn in artificial samples(Cu:Zn=0.03:0.5～04:0. 02μg/25mL)and in human serumsamples,and the relative errors for the former arewithin±5%(4th-derivative)and the results for thelatter are in good agreement with those of atomicabsorption spectrometry.
出处
《分析试验室》
CAS
CSCD
北大核心
1994年第6期11-14,共4页
Chinese Journal of Analysis Laboratory
关键词
卟啉
分光光度法
血清
铜
锌
Derivative spectrophotometry
partialleast squares method
porphyrin
copper,zincdetermination:serun.
