摘要
本文报道Nocardia sp.48(Str^r Rif^s)和Nocardia sp.189(Str^sRif^r)原生质体融合结果。42%PEG6000获最高融合频率。经一定时间的表型延迟后检出抗性互补的融合子。用电子显微镜观察了原生质体融合过程。融合子形态多样,耐双药性状稳定。有四株融合子产生亲本没有的甾体转化中间体,三株融合子产生亲本没有的抗生物质,还得到一株甾体转化活力明显高于亲本的融合子。
This paper reports the result of recombination by protoplast fusion between Nocardia sp.48 (Str^r Rif^s) and Nocardia sp.189 (Str^s Rif^r). The maximum recombinant frequency reached 4.39×10^(-3) by treatment of 42% polyethylene glycol 6000. Fusants could grow only if Streptomycin and Rifampin were added to the regeneration medium after waiting the phenotypie lag. The processes of protoplast fusion was observed with electrort microscope.
Besides a variety of colony shape, Four fusants produced a new intermediate compound of steroid transformation. Three fusants generated antibiotic substances, which their parents didn't produced. We also obtain a fusant whose steroid converting rate was obviously higher than its parants.
出处
《生物工程学报》
CAS
CSCD
北大核心
1989年第4期303-308,共6页
Chinese Journal of Biotechnology
