摘要
应用斑点免疫金银染色技术(Dot-IGSS),以华支睾吸虫成虫抗原(AWA)、尿素溶解性成虫抗原(CWU)和硫酸铵沉淀成虫抗原(AW)诊断华支睾吸虫病,60例血清抗体检出率均达100.0％,与正常人血清反应(60例)阴性符合率为93.3～96.6％,与日本血吸虫病人(60例)的交叉反应为0～5.0％,与囊虫病人(30例)和肝炎病人(15例)的交叉反应分别为3.3～6.7％和0。各抗原间未见显著性差异(P>0.05)。但与混合阳性血清反应,AW活性似较AWA和CWU强。
Three antigens of Clonorchis sinensis,AWA(adult worm antigen) ,CWU(urea-soluble worm antigen from C. sinensis) and AW(amonium sulfate precipitable worm antigen) ,were compared for diagnosing clonorchiasis by dot-immunogold-silver staining, of 60 proven clonorchiasis cases,the positive rates were all 100. 0%. The negative rates for 60 healthy individuals were 93. 3-96. 6%. And the cross reaction rates for 60 schistosomiasis,30 cysticercosis and 15 hepatitis patients were 0-5. 0% ,3. 3-6. 7% and 0, respectively. No significant differences were found among the 3 antigens (P>0. 05). But the titer of AW reacted with the mixed-positive sera was higher than those of AWA and CWU.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1993年第5期25-27,共3页
Chinese Journal of Zoonoses
关键词
抗原
异形吸虫病
免疫诊断
Antigen
Clonorchis sinensis
Dot-immunogold-silver staining (Dot-IGSS).
