摘要
论述了魔芋细胞组织培养研究方面所取得的成果与进展,如芽在MS+NAA 0.3-0.5mg／L生根的培养基,生根率达到100％等,并针对当前在魔芋组织培养中存在的突出问题如由于多酚氧化物的氧化而产生的细胞的褐变现象以及组培苗的玻璃化等现象做出了分析:如开始时置于暗处培养可以防止或减少褐变现象,加入GA3等微量元素于培养基中可以减少组培苗的玻璃化的发生。这些均为下一步魔芋组培快繁技术应用于生产提供理论依据。
The article discusses the latest results and research progresses on the tissue culture of Amorphophallus conjac, such as all the regenerated plantlets rooted readily on MS + NAA 0.3- 0.5mg/L and grow into complete plants, which are based on the results of plant tissue culture in this field. It analyses the main problems of the current study on tissue culture of Amorphophallus conjac, such as the browning of media for the oxidation of polyphenol oxide and vetrification. It is better that the materials are cultured in the darkness for the browning of media and it can decrease vetrification if there is trace element such as GA3 in the culture medium. It will benefit for further research on proliferation for production practice.
出处
《中国农学通报》
CSCD
2005年第1期153-155,共3页
Chinese Agricultural Science Bulletin
基金
云南省科技攻关项目资助。
