摘要
目的 研究HPV16型病毒感染与食管癌发生的关系。方法 包装含有HPV16E6E7基因重组逆转录病毒 ,以重组病毒感染人胚食管纤维细胞 ,在TPA协同下诱导SCID小鼠成瘤。结果 重组病毒感染人胚食管纤维细胞可以诱导SCID小鼠形成肉瘤 ,可以检测到E6E7基因的存在及表达 ,流式细胞仪检测从瘤组织培养出来的纤维细胞 ,确定为异倍体 ;但未能诱导人胚食管组织成瘤。结论 建立的重组逆转录病毒系统可以成功介导HPV16E6E7基因的转移 ,可以应用于HPV致瘤性研究。
Objective To study the etiological role of human papillomavirus type 16 (HPV16) infection in the development of esophageal cancers Methods A recombinant retrovirus containing the E6E7 ORFs of HPV16 was packaged and human fetal esophageal fibroblasts were infected The tumorigenecity of the fibroblasts was tested in SCID mice in synergy with 12-O-tetradecanoylphorbol-13-acetate (TPA) Results Human esophageal fibroblasts infected with the recombinant retrovirus induced sarcomas in SCID mice, the existence and expression of E6E7 ORFs was confirmed in the sarcomas Fibroblasts cultured from the sarcoma were demonstrated heteroploid by cytoflowmetry However, tumors were not observed in human fetal esophagus infected with such virus Conclusion These results revealed that the established recombinant retroviral system can successfully mediate the transference of HPV16 E6E7 genes, and such system is applicable to researches on tumorigenesis of HPV
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2004年第3期223-226,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金重点资助项目 ( 3 983 0 3 80 )
