摘要
目的 :探讨壳聚糖纤维与体外培养Schwann细胞的相容性和亲和力。方法 :新生SD鼠的臂丛和坐骨神经组织块种植于壳聚糖纤维支架上培养Schwann细胞。结果 :壳聚糖纤维支架上的Schwann细胞为橄榄形或椭圆形 ,细胞在纤维上分裂、迁移 ,形成链状结构 ,细胞突起的末端呈扁平状膨大 ,伸出爪形伪足贴附于纤维。结论 :壳聚糖纤维不仅与Schwann细胞的相容性良好 。
Objective: To explore the histocompatibility of the chitosan fiber with Schwann cells in vitro. Methods: Schwann cells migrated from brachial plexus or sciatic nerve piece of new born Sprague-Dawley rats were cultured on the chitosan fiber. Results: Schwann cells cultured on the chitosan fiber were spindle or oval-shaped, proliferated and migrated along fiber and formed chain structure. The end of Schwann cell processes were flat and expanded,adhered on the fiber as claw-shaped pseudopodium. Conclusion: There is good histocompatibity between the chitosan fiber and Schwann cell. The chitosan fiber possibly induces Schwann cell to form the myelin sheath in vitro.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2004年第6期630-633,共4页
Chinese Journal of Anatomy
基金
上海市高等学校科学技术发展基金项目 (0 3BK15 )
上海市教委第四期重点学科资助项目 (ZDXK2 0 0 1)
