摘要
目的 研究三氧化二砷对恶性淋巴瘤细胞辐射敏感性的影响。方法 采用细胞染色方法观察细胞凋亡的形态 ,流式细胞仪检测细胞的DNA含量和Bcl 2蛋白的表达。结果 0 5～ 2 0μmol L三氧化二砷和 1～ 4Gyγ射线均能抑制B淋巴瘤细胞株Raji细胞生长 ,诱导细胞凋亡 ,流式细胞仪检测Raji细胞亚G1 期细胞明显增多 ,Bcl 2蛋白的表达明显降低 ,呈现时间剂量依赖效应 ,联合应用比单独应用抑制作用显著。结论 三氧化二砷能够提高恶性淋巴瘤细胞的放射敏感性。
Objective To investigate the radiosensitization of arsenic trioxide on malignant lymphoma cell lines. Methods Cell apoptosis situation, cellular DNA contents and Bcl-2 protein expression were determined by cell staining and flow cytometry. Results One to four Gy 60Co γ-irradiation and 0.5- 2.0 μmol/L As 2O 3 could inhibit Raji cell growth accompanied by the appearance of morphologic characteristics of apoptosis. The number of Sub-G 1 cells significantly increased and Bcl-2 protein expression in Raji cells was significantly downregulated as assayed by flow cytometry. The relationship between Bcl-2 protein the concentration of As 2O 3 and γ-radiation showed both dose- and time-dependent features; so did the number of Sub-G 1 cells. The inhibitory effect was much more evident when As 2O 3 and irradiation were applied combinatively than either one of them was used alone. Conclusion Arsenic trioxide is able to enhance radiation effect on malignant lymphoma cell lines. \;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2005年第1期43-45,共3页
Chinese Journal of Radiological Medicine and Protection
基金
广东省医学科学技术研究基金资助项目 (B2 0 0 2 0 69)
