摘要
目的 探讨全脑缺血 再灌流时葛根素的脑保护作用机制。方法 将实验大鼠随机分为空白对照 (S)组、全脑缺血 再灌流 (IR)组、葛根素 (P)组。采用Pulsinelli法制备大鼠全脑缺血 再灌流损伤模型 ,以原位杂交法分别测定全脑缺血 再灌流 2 ,6 ,12 ,2 4 ,4 8h各时点海马CA1 区肿瘤坏死因子 α (TNF α)mRNA、白介素 1β (IL β)mRNA的表达 ,HE染色光镜下计数存活神经元数的变化。 结果 与IR组相比 ,P组各对应时点TNF αmRNA、IL 1βmRNA的表达均有显著下降 (P <0 0 5 ) ,而存活神经元数目均有明显增加 (P <0 0 1或P <0 0 5 )。结论 葛根素通过下调TNF αmRNA、IL 1βmRNA的表达 。
Objective To explore the protective mechanism of Puerarin on brain after global cerebral ischemia-reperfusion.Methods The rats were randomly divided into three groups:sham operation group(S group),only exposed anatomically without cerebral ischemia and reperfusion ;ischemia and reperfusion group(IR group),global cerebral ischemia and reperfusion with administration of equal volume of normal saline through abdomen;Puerarin group(P group),global cerebral iscbemia and reperfusion with administration of Puerarin through abdomen.Global cerebral ischemia-reperfusion model was induced by means of Pulsmelli's four vessels occlusion method.The expressions of TNF-α mRNA and IL-1β mRNA were determined by In situ hybridization at 2 h,6 h,12 h,24 h,48 h after global cerebral ischemia-reperfusion.The number of surviving neurons was calculated through Olympus microscopy after HE staining in hippocampal CA 1 region tissues.Results Compared with IR group,the expressions of TNF-α mRNA and IL-1 βmRNA in P group were significantly decreased(P<0.01 and P<0.05),and the number of surviving neurons was obvisously increased(P<0.01 and P<0.05).Conclusion The protective effect of Puerarin on rat brain might be associated with decreased expressions of TNF-α mRNA and IL-1β mRNA after global cerebral ischemia-reperfusion.
出处
《中华急诊医学杂志》
CAS
CSCD
2005年第2期119-121,共3页
Chinese Journal of Emergency Medicine
基金
湖北省科技攻关计划项目 ( 2 0 0 3AA3 0 1C5 1)
