摘要
用浸根接种法对来自全国八省(市)、自治区的西瓜枯萎病菌(Fusarium oxysporium f. sp. niveum, FON)10个分离物进行了生理小种分化鉴定研究。根据这10个分离物在6个鉴别寄主上的反应,初步把它们划分为小种0,1,2,3,计4个生理小种。同时对这10个西瓜枯萎病菌分离物(FON isolates)的菌丝体进行了过氧化物酶同工酶(POD)聚丙烯酰胺凝胶电泳分析。电泳图谱的分析结果支持了浸根接种鉴定的结论。
Ten isolates of Fusarium oxysporum f. sp. niveum (FON) from eight provinces (cities or autonomous regions) in China are identified by root dip inoculation. Based on the reactions of the ten isolates on six differential host, four physiological races, Race 0, Race 1, Race 2 and Race 3 are suggested primarily. The peroxidase (POD) isoenzymic polyacrylamide gel electrophoresis of the mycelia of the ten isolates are analysed. The POD isoenzyme zymograms of the isolates support above result. The identification method of physiological race differentiation of FON isolates in China was discussed as well.
出处
《西北农业学报》
CAS
CSCD
1993年第4期67-70,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金
关键词
西瓜
枯萎病
生理小种分化
Fusarium oxysporum f. sp. niveum
Physiological race differentiation
Isoenzyme eletrophoresis